Day7 final day of JAPOHR training in Japan

Day 7 (30th Aug 2018)

Courtesy court to Director of NIID: Dr Wakita

Discussion with Dr Inoue 

Discussion about the autopsy of rabies case imported cases 

Lecture about Rabies Vaccine 

Lecture by Sheila and Joley 

Farewell, party and certificate mini ceremony in Izakaya and Karaoke! 

Congratulation to all participants! 

Training day 6

Day 6 (29th Aug 2018)

Tour in National Institute of infectious diseases(NIID) and the laboratories. 

Courtesy court to director of the department of Veterinary Science: Dr Shigeru Morikawa

Short lecture and observation of electron microscope by Dr Hasegawa

Move to Murayama branch in NIID

Tour in Division of biosafety control and research (BSL 4 Laboratory)  with Dr Iki 

Lecture of activities of the department of virology department and SFTS byDr Shimojima

Lecture of diagnosis electron microscopy of the virus by Dr Nagata and tour of the pathological department

Training Day 5

Day5 (27th Aug 2018)

Practice of RAPINA

Discussion of the overall project and each role

Whole slide imaging scanner. 

This day was last day staying at Kitazato University. 

Great hospitality and warm teaching. 

Great appreciate to Prof Baku, Shiwa and students in veterinary pathology, school of veterinary medicine, faculty of veterinary medicine in Kitazato University

Weekend

Weekend Tour around Towada and Hachinoye 

We went to Kabushima, Ashigezaki, Tanesashi and also shopping!!! 

Then Dr Balu invited us to his house. There is a large garden with many animals there. it's like a farm! 

We ate homemade "kimuchi". it was so delicious. 

Thank you so much for your great hospitality.

Training Day 4

Day 4 (24th Aug 2018) 

Discussion about SATREPS

Observation of histopathological appearance of dog rabies by fluorescence microscopy. 

Training of RAPINA (Rapid diagnostic test for Rabies antibody test)

Observation of histopathological appearance of dog rabies by Transmission Electron Microscopy：TEM

Lectures by Sheilla (RITM) and  Joely (RADDLE3)

Training Day3

Day 3 (23rd Aug 2018)

Trainees learned how to make IHC (Immunohistochemistry) slide 

and how to read IHC slide

Dr Baku taught how to read rabies cases in dog and human 

There was an autopsy case of a dog

We could observe dog necropsy. (necropsy diagnosis: hemangiosarcoma) 

And performed sampling of follicle sinus complex

Training Day 2

Day 2 (22nd Aug 2018) 

Histopathology training, hands-on training using real animal samples
HE slides observation of rabid dogs and human cases
Preparation of IHC slides of dog brain, salivary gland, skin tissues

ICT(immunochromatographic test) training was also conducted. 

ICT (Rabies antigen kit) was invented by Pro Nishizono (Principal investigator) 

In this project, we want to evaluate efficacies to diagnosis rabies including human and dogs. This time, we use brain and muzzle hair samples for ICT. 

The trainees learned how to use this kit and the mechanizm. 

After the trainig, staff and students of Kitazato Uni prepared BBQ. We enjoyed it!